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Constant heat stress reduces skeletal muscle protein deposition in broilers
Author(s) -
Zuo Jianjun,
Xu Mei,
Abdullahi Yusuf Auwalu,
Ma Limei,
Zhang Zhongyue,
Feng Dingyuan
Publication year - 2014
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.6749
Subject(s) - endocrinology , medicine , skeletal muscle , transaminase , hsp70 , heat shock protein , chemistry , biology , zoology , biochemistry , enzyme , gene
BACKGROUND This experiment was conducted to evaluate the effects of constant heat stress on growth performance and protein metabolism in skeletal muscle of Arbor Acres broilers. RESULTS Two hundred and seventy 21‐day‐old Arbor Acres broilers with similar body weight (1298 ± 28 g) were selected for a 3‐week trial (29–49 days of age). The broilers were randomly assigned to three groups including the control group, constant heat stress group and pair‐fed group. Up‐regulation of the rectal temperature and the mRNA expression of heat shock protein 70 in liver indicate that the model for constant heat stress was success. The average daily gain, feed conversion ratio, breast and thigh muscle weight, percentage of breast muscle, crude protein content in breast and thigh muscle in constant heat stress group were significantly lower than in control group and pair‐fed group. Serum uric acid content and the glutamic‐oxaloacetic transaminase activity were significantly higher, while protein content and glutamic‐pyruvate transaminase activity were significantly lower in liver of heat stress group than of the control and pair‐fed groups. The expression of insulin‐like growth factor 1, phosphatidylinositol 3‐kinase and p70S6 kinase associated with protein synthesis were lower in breast muscle but higher in thigh muscle in heat stress group compared to the control or fed‐pair groups. In thigh muscles, the expression of muscle ring‐finger protein‐1 and MAFbx associated with protein degradation were higher in the heat stress group than in the control and pair‐fed groups. CONCLUSION Poor performance of the birds under heat stress may be due to lower synthesis and increased degradation of proteins. © 2014 Society of Chemical Industry