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Antiproliferative and immunostimulatory activity of a protein from Pleurotus eryngii
Author(s) -
Mariga Alfred Mugambi,
Yang Wenjian,
Mugambi Dorothy Kemuma,
Pei Fei,
Zhao Liyan,
Shao Yani,
Hu Qiuhui
Publication year - 2014
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.6665
Subject(s) - cytotoxicity , pleurotus eryngii , chemistry , cell growth , pharmacology , in vitro , biochemistry , biology , cancer research , food science , mushroom
BACKGROUND Currently, the use of mushrooms as functional foods, nutraceuticals or phytopharmaceuticals source has risen. In contrast, the possible cellular cytotoxicity and immunostimulatory activity of Pleurotus eryngii ( DC . ex Fr.) Quel protein ( PEQP ) is unknown. Here we report extraction, anti‐tumorigenic and immunostimulatory activity of PEQP in vitro . RESULTS PEQP was extracted from the dried fruiting bodies of P. eryngii , purified and characterised. Its in vitro antiproliferative activity was then evaluated in human non‐small cell lung cancer A549 ( NSCLC ), stomach adenocarcinoma BGC ‐823, hepatocellular carcinoma HepG2 and gastric carcinoma HGC ‐27 cell lines using conventional cancer drugs (paclitaxel, doxorubicin and mitomycin C) as positive controls. The protein fractions ( PEQP 1, 2, 3 and 4) obtained inhibited tumour cell proliferation dose‐dependently with fraction PEQP 2 having significant ( P < 0.05) toxicity in all tumour cells. PEQP had no significant toxicity on normal liver Chang cells but their proliferation was significantly inhibited by mitomycin C. Moreover, PEQP stimulated the proliferation, lysosomal enzyme activity, pinocytosis, nitric oxide and hydrogen peroxide production of RAW 264.7 cell lines dose‐dependently. CONCLUSION Based on these results, P. eryngii protein has a potential application in functional foods as a natural anti‐tumour agent with immunostimulatory activity. © 2014 Society of Chemical Industry