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Screening DNA chip and event‐specific multiplex PCR detection methods for biotech crops
Author(s) -
Lee SeongHun
Publication year - 2014
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.6625
Subject(s) - canola , multiplex , multiplex polymerase chain reaction , microbiology and biotechnology , biology , genetically modified organism , polymerase chain reaction , dna microarray , gene , food science , genetics , gene expression
Abstract BACKGROUND There are about 80 biotech crop events that have been approved by safety assessment in Korea. They have been controlled by genetically modified organism (GMO) and living modified organism (LMO) labeling systems. The DNA‐based detection method has been used as an efficient scientific management tool. Recently, the multiplex polymerase chain reaction (PCR) and DNA chip have been developed as simultaneous detection methods for several biotech crops' events. RESULTS The event‐specific multiplex PCR method was developed to detect five biotech maize events: MIR604, Event 3272, LY 038, MON 88017 and DAS‐59122‐7. The specificity was confirmed and the sensitivity was 0.5%. The screening DNA chip was developed from four endogenous genes of soybean, maize, cotton and canola respectively along with two regulatory elements and seven genes: P35S, tNOS, pat , bar , epsps1 , epsps2 , pmi , cry1Ac and cry3B . The specificity was confirmed and the sensitivity was 0.5% for four crops' 12 events: one soybean, six maize, three cotton and two canola events. CONCLUSION The multiplex PCR and DNA chip can be available for screening, gene‐specific and event‐specific analysis of biotech crops as efficient detection methods by saving on workload and time. © 2014 Society of Chemical Industry