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Quercetin‐rich onion peel extract suppresses adipogenesis by down‐regulating adipogenic transcription factors and gene expression in 3T3‐L1 adipocytes
Author(s) -
Bae ChoRong,
Park YunKyung,
Cha YounSoo
Publication year - 2014
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.6604
Subject(s) - adipogenesis , quercetin , 3t3 l1 , intracellular , lipolysis , chemistry , biochemistry , triglyceride , viability assay , biology , adipose tissue , cholesterol , cell , antioxidant
BACKGROUND Onion peel contains a high amount of quercetin, which has been reported to have anti‐cholesterol, antithrombotic and insulin‐sensitizing properties. This study aimed to elucidate the anti‐adipogenic effects of quercetin‐rich onion peel extract ( OPE ) and to compare it with commercially available quercetin using 3T3‐L1 preadipocytes.RESULTS Without affecting cell viability, both OPE and quercetin averted adipogenesis, as characterized by dose‐dependent decreases in intracellular triglyceride content and glycerol 3‐phosphate dehydrogenase activity, but the effect was more pronounced with OPE than with quercetin. The mRNA expression levels of key adipogenic genes such as PPAR γ , C/ EBP α , FABP4 , aP2 and LPL were decreased in a dose‐dependent manner by both OPE and quercetin.CONCLUSION The results indicate that OPE treatment significantly prevents intracellular lipid accumulation via hyperactivation of genes regulating lipolysis as compared with quercetin alone. © 2014 Society of Chemical Industry