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Anthocyanin profiling of wild maqui berries ( Aristotelia chilensis [Mol.] Stuntz) from different geographical regions in Chile
Author(s) -
Fredes Carolina,
Yousef Gad G,
Robert Paz,
Grace Mary H,
Lila Mary Ann,
Gómez Miguel,
Gebauer Marlene,
Montenegro Gloria
Publication year - 2014
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.6602
Subject(s) - anthocyanin , biology , genotype , botany , food science , population , horticulture , genetics , gene , demography , sociology
BACKGROUND Maqui ( Aristotelia chilensis ) is a Chilean species which produces small berries that are collected from the wild. Anthocyanins, because of their health benefits, are the major focus of interest in maqui fruit. For this study, we examined anthocyanin and phenolic content of maqui fruits from individuals that belonged to four geographical areas in Chile, and used DNA marker analysis to examine the genetic variability of maqui populations that had distinctly different fruit anthocyanin content . RESULTS Twelve primers generated a total of 145 polymorphic inter simple sequence repeat–polymerase chain reaction ( ISSR‐PCR ) bands. ISSR‐PCR showed different banding patterns for the individuals evaluated, confirming that maqui populations belonged to different genotypes. Maqui fruit from four different geographical regions during two consecutive growing seasons showed high total anthocyanin (6.6–15.0 g cy‐3‐glu kg −1 fresh weight ( FW )) and phenolic (10.7–20.5 g GAE kg ‐1 FW ) contents and different anthocyanin profiles . CONCLUSION Three maqui genotypes exhibited significantly higher anthocyanin content than the others, as measured by pH differential method and high‐performance liquid chromatography–mass spectrometry. Significant genetic diversity was noted within each ecological population. ISSR‐PCR analysis provided a fingerprinting approach applicable for differentiation of maqui genotypes. © 2014 Society of Chemical Industry