Premium
Association of protein expression in isolated milk epithelial cells and cis ‐9, trans ‐11 conjugated linoleic acid proportions in milk from dairy cows
Author(s) -
Wang Tao,
Lim JiNa,
Bok JinDeok,
Kim JeongHoon,
Kang SangKee,
Lee SangBum,
Hwang JinHee,
Lee KyungHoon,
Kang HanSeok,
Choi YunJaie,
Kim EunJoong,
Choi SeongHo,
Lee HongGu
Publication year - 2014
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.6502
Subject(s) - conjugated linoleic acid , casein , biology , food science , linoleic acid , messenger rna , biochemistry , chemistry , gene , fatty acid
BACKGROUND The effects of the individual variation among dairy cows on the synthesis of cis ‐9, trans ‐11 conjugated linoleic acid ( CLA ) are still not well characterised. Therefore, the protein expression profiles of isolated milk epithelial cells ( MECs ) were detected by two‐dimensional electrophoresis and their correlation with the various proportion of cis ‐9, trans ‐11 CLA were evaluated . RESULTS Although animals were offered the same diet, the proportion of cis ‐9, trans ‐11 CLA in group High (1.02 ± 0.10%) was twice as high as that in group Low (0.59 ± 0.14%) ( P < 0.05). MECs with the characteristics of native epithelial cells were successfully isolated from the milk and these cells had no obvious RNA degradation or were hardly contaminated with leucocytes or blood red cells. Moreover, the protein expression pattern of cathelicidin 5 in isolated MECs was positive, whereas annexin I (confirmed by real‐time polymerase chain reaction), ZW10 interactor and κ ‐casein were negatively related to the proportion of cis ‐9, trans ‐11 CLA in the milk fat . CONCLUSION The varied individual content of cis ‐9, trans ‐11 CLA in cows may be associated with annexin I. These findings may provide some theoretical basis for studies concerning the effects of the individual variation among dairy cows of the synthesis of cis ‐9, trans ‐11 CLA . © 2013 Society of Chemical Industry