Genetic assessment of common bean ( Phaseolus vulgaris L.) accessions by peroxidase gene‐based markers

BACKGROUND Peroxidase, a plant‐specific oxidoreductase, is a heme‐containing glycoprotein encoded by a large multigenic family in plants. Plant peroxidases ( POXs , EC 1.11.1.7) play important roles in many self‐defense interactions in plants. Here, 67 common bean ( Phaseolus vulgaris L.) genotypes were studied using a POX gene‐based marker method. Comparison of POX genes could resolve evolutionary relationships in common bean . RESULTS Eighty fragments were obtained with 20 primer pairs that amplified one ( POX8c ) to eight ( ATP29 ) bands, with a mean of four bands per primer pair. The average (polymorphic information content) PIC value for the POX products was 0.40. The maximum variation (93%) was found between Turkey (#33) and India (#52) and between Antalya (#33) and India (#53). The minimum variation (0%) was found among four pairs: Bozdag (#2) and Karadeniz (#38), Kirklareli (#11) and Turkey (#15, 16, 43), Bandirma (#13) and Turkey (#15, 16, 43), and Kirklareli (#10) and Bandirma (#22). UPGMA was used to discriminate the common bean genotypes into five clusters, while STRUCTURE software was used to investigate the genetic population structure . CONCLUSION The results showed that POX gene family markers can be used to study genotypic diversity and provide new information for breeding programs and common bean improvement practices. © 2013 Society of Chemical Industry