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Genetic assessment of common bean ( Phaseolus vulgaris L.) accessions by peroxidase gene‐based markers
Author(s) -
Nemli Seda,
Kaya Hilal Betul,
Tanyolac Bahattin
Publication year - 2014
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.6477
Subject(s) - phaseolus , upgma , biology , genetic diversity , genotype , genetic variation , botany , gene , genetics , horticulture , population , demography , sociology
Abstract BACKGROUND Peroxidase, a plant‐specific oxidoreductase, is a heme‐containing glycoprotein encoded by a large multigenic family in plants. Plant peroxidases ( POXs , EC 1.11.1.7) play important roles in many self‐defense interactions in plants. Here, 67 common bean ( Phaseolus vulgaris L.) genotypes were studied using a POX gene‐based marker method. Comparison of POX genes could resolve evolutionary relationships in common bean . RESULTS Eighty fragments were obtained with 20 primer pairs that amplified one ( POX8c ) to eight ( ATP29 ) bands, with a mean of four bands per primer pair. The average (polymorphic information content) PIC value for the POX products was 0.40. The maximum variation (93%) was found between Turkey (#33) and India (#52) and between Antalya (#33) and India (#53). The minimum variation (0%) was found among four pairs: Bozdag (#2) and Karadeniz (#38), Kirklareli (#11) and Turkey (#15, 16, 43), Bandirma (#13) and Turkey (#15, 16, 43), and Kirklareli (#10) and Bandirma (#22). UPGMA was used to discriminate the common bean genotypes into five clusters, while STRUCTURE software was used to investigate the genetic population structure . CONCLUSION The results showed that POX gene family markers can be used to study genotypic diversity and provide new information for breeding programs and common bean improvement practices. © 2013 Society of Chemical Industry