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Enhanced biomass delignification and enzymatic saccharification of canola straw by steam‐explosion pretreatment
Author(s) -
Garmakhany Amir Daraei,
Kashaninejad Mahdi,
Aalami Mehran,
Maghsoudlou Yahya,
Khomieri Mortza,
Tabil Lope G
Publication year - 2014
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.6466
Subject(s) - steam explosion , hemicellulose , enzymatic hydrolysis , xylose , cellulose , chemistry , lignocellulosic biomass , biomass (ecology) , canola , hydrolysis , bioconversion , ethanol fuel , pulp and paper industry , straw , biofuel , biorefinery , food science , agronomy , biochemistry , fermentation , organic chemistry , microbiology and biotechnology , raw material , biology , inorganic chemistry , engineering
BACKGROUND In recent decades, bioconversion of lignocellulosic biomass to biofuel (ethanol and biodiesel) has been extensively investigated. The three main chemical constituents of biomass are cellulose, hemicellulose and lignin. Cellulose and hemicellulose are polysaccharides of primarily fermentable sugars, glucose and xylose respectively. Hemicellulose also includes small fermentable fractions of arabinose, galactose and mannose. The main issue in converting lignocellulosic biomass to fuel ethanol is the accessibility of the polysaccharides for enzymatic breakdown into monosaccharides. This study focused on the use of steam explosion as the pretreatment method for canola straw as lignocellulosic biomass . RESULTS Result showed that steam explosion treatment of biomass increased cellulose accessibility and it hydrolysis by enzyme hydrolysis. Following 72 h of enzyme hydrolysis, a maximum cellulose conversion to glucose yield of 29.40% was obtained for the steam‐exploded sample while the control showed 11.60% glucose yields. Steam explosion pretreatment increased glucose production and glucose yield by 200% and 153.22%, respectively, compared to the control sample. The crystalline index increased from 57.48% in untreated canola straw to 64.72% in steam‐exploded samples . CONCLUSION Steam explosion pretreatment of biomass increased cellulose accessibility, and enzymatic hydrolysis increased glucose production and glucose yield of canola straw. © 2013 Society of Chemical Industry