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Scutellaria polysaccharide inhibits the infectivity of Newcastle disease virus to chicken embryo fibroblast
Author(s) -
Xiaona Zhao,
Jianzhu Liu
Publication year - 2013
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.6440
Subject(s) - infectivity , polysaccharide , newcastle disease , virus , scutellaria , fibroblast , chemistry , microbiology and biotechnology , biology , virology , biochemistry , in vitro , medicine , alternative medicine , traditional chinese medicine , pathology
BACKGROUND To select the antiviral active site of Scutellaria polysaccharide ( SPS ), safe concentrations of crude total Scutellaria polysaccharide ( SPS t ) and fractional polysaccharide SPS 50 , SPS 60 , SPS 70 and SPS 80 on chicken embryo fibroblast ( CEF ) were first compared using the MTT method. Then, SPS t , SPS 50 , SPS 60 , SPS 70 , and SPS 80 at five concentrations within the safe concentration, together with Newcastle disease virus ( NDV ), were added to the cultivating system of CEF in three models: pre‐addition of polysaccharide, post‐addition of polysaccharide, and simultaneous addition of polysaccharides and NDV after mixing. The effects of SPS on the cellular infectivity of NDV ( A 570 value and the highest viral inhibitory rate) were compared using the MTT method.RESULTS At appropriate concentrations, the five polysaccharides could significantly inhibit the infectivity of NDV on CEF. Among the five polysaccharide groups, the SPS 80 group exhibited the highest viral inhibitory rate in the three sample‐addition modes.CONCLUSION This finding indicates that SPS 80 possesses the best efficacy as a component of antiviral polysaccharide drug. © 2013 Society of Chemical Industry