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Cow's milk with active immunoglobulins against Campylobacter jejuni : Effects of temperature on immunoglobulin activity
Author(s) -
Riera Francisco,
Alvarez Alejandro,
Espi Alberto,
Prieto Miguel,
de la Roza Begoña,
Vicente Fernando
Publication year - 2014
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.6398
Subject(s) - pasteurization , antibody , campylobacter jejuni , campylobacter , food science , chemistry , antiserum , cow milk , zoology , biology , immunology , bacteria , genetics
BACKGROUND Adult Holstein cows were injected with an antiserum against Campylobacter jejuni and immunoglobulin activities in vitro were determined in blood and milk several weeks after injection. The immunoactivity of immunoglobulins in milk was measured by an ELISA after different temperature–time treatments (60–91°C and 4–3600 s) at laboratory and pilot‐plant scales. Kinetic and thermodynamic parameters were determined . RESULTS An increase in immunoglobulin activity in milk was detected several days after injection. Optical densities increased by three‐ to seven‐fold in this period. The activity started to decay 4–5 weeks after injection. Immunoglobulins maintained most of their in vitro activity under pasteurisation conditions (72°C and 15 s) and were denatured following first‐order kinetics . CONCLUSIONS The injection protocol applied allows milk with specific immunoglobulins against Campylobacter jejuni to be obtained. Traditional pasteurisation did not reduce this activity. © 2013 Society of Chemical Industry