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Fatty acid composition, physicochemical properties, antioxidant and cytotoxic activity of apple seed oil obtained from apple pomace
Author(s) -
Walia Mayanka,
Rawat Kiran,
Bhushan Shashi,
Padwad Yogendra S,
Singh Bikram
Publication year - 2013
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.6337
Subject(s) - pomace , food science , composition (language) , chemistry , antioxidant , chemical composition , botany , biology , biochemistry , organic chemistry , philosophy , linguistics
BACKGROUND Apple pomace is generated in huge quantities in juice‐processing industries the world over and continuous efforts are being made for its inclusive utilization. In this study, apple seeds separated from industrial pomace were used for extraction of oil. The fatty acid composition, physicochemical and antioxidant as well as in vitro anticancer properties of extracted oil were studied to assess its suitability in food and therapeutic applications.RESULTS The fatty acid composition of seed oil revealed the dominance of oleic (46.50%) and linoleic acid (43.81%). It had high iodine (121.8 g I 100 g −1 ) and saponification value (184.91 mg KOH g −1 oil). The acid value, refractive index and relative density were 4.28 mg KOH g −1 , 1.47 and 0.97 mg mL −1 , respectively. The antioxidant potential ( IC 50 ) of apple seed oil was 40.06 µg mL −1 . Cytotoxicity of apple seed oil against CHOK1 , SiHa and A549 cancer cell lines ranged between 0.5 ± 0.06% and 88.6 ± 0.3%.CONCLUSION The physicochemical properties of apple seed oil were comparable with edible food oil, indicating its better stability and broad application in the food and pharmaceutical industries. Apple seed oil could be a good source of natural antioxidants. Also, the in vitro cytotoxic activity against specific cell lines exhibited its potential as an anticancer agent. © 2013 Society of Chemical Industry