Premium
A liquid chromatography/electrospray ionisation tandem mass spectrometry method for the simultaneous quantification of salicylic, jasmonic and abscisic acids in Coffea arabica leaves
Author(s) -
de Sá Marta,
Ferreira João P,
Queiroz Vagner T,
VilasBoas Luís,
Silva Maria C,
Almeida Maria H,
GuerraGuimarães Leonor,
Bronze Maria R
Publication year - 2014
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.6288
Subject(s) - jasmonic acid , chromatography , coffea arabica , chemistry , salicylic acid , electrospray , mass spectrometry , abscisic acid , tandem mass spectrometry , liquid chromatography–mass spectrometry , electrospray ionization , biochemistry , botany , biology , gene
BACKGROUND Plants have developed an efficient system of recognition that induces a complex network of signalling molecules such as salicylic acid ( SA ), jasmonic acid ( JA ) and abscisic acid ( ABA ) in case of a pathogenic infection. The use of specific and sensitive methods is mandatory for the analysis of compounds in these complex samples . RESULTS In this study a liquid chromatography/electrospray ionisation tandem mass spectrometry method was developed and validated for the simultaneous quantification of SA , JA and ABA in Coffea arabica (L.) leaves in order to understand the role of these phytohormones in the signalling network involved in the coffee defence response against Hemileia vastatrix . The results showed that the method was specific, linear ( r ≥ 0.99) in the range 0.125–1.00 µg mL −1 for JA and ABA and 0.125–5.00 µg mL −1 for SA , and precise (relative standard deviation ≤11%), and the limit of detection (0.010 µg g −1 fresh weight) was adequate for quantifying these phytohormones in this type of matrix . CONCLUSION In comparison with healthy leaves, those infected with H. vastatrix (resistance reaction) displayed an increase in SA level 24 h after inoculation, suggesting the involvement of an SA ‐dependent pathway in coffee resistance. © 2013 Society of Chemical Industry