Premium
Metabolite profiling and quantification of phytochemicals in potato extracts using ultra‐high‐performance liquid chromatography–mass spectrometry
Author(s) -
Chong Esther Swee Lan,
McGhie Tony K,
Heyes Julian A,
Stowell Kathryn M
Publication year - 2013
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.6285
Subject(s) - metabolite , metabolomics , chemistry , hydroxycinnamic acid , mass spectrometry , solanum tuberosum , flavonols , chromatography , cultivar , metabolite profiling , liquid chromatography–mass spectrometry , flavonoid , botany , biology , biochemistry , antioxidant
BACKGROUND Potatoes contain a diverse range of phytochemicals which have been suggested to have health benefits. Metabolite profiling and quantification were conducted on plant extracts made from a white potato cultivar and ‘Urenika’, a purple potato cultivar traditionally consumed by New Zealand Maori. There is limited published information regarding the metabolite profile of Solanum tuberosum cultivar ‘Urenika’ . RESULTS Using ultra‐high‐ performance liquid chromatography–mass spectrometry ( UHPLC‐MS ), a total of 31 compounds were identified and quantified in the potato extracts. The majority of the compounds were identified for the first time in ‘Urenika’. These compounds include several types of anthocyanins, hydroxycinnamic acid ( HCA ) derivatives, and hydroxycinnamic amides ( HCAA ). Six classes of compounds, namely organic acids, amino acids, HCA , HCAA , flavonols and glycoalkaloids, were present in both extracts but quantities varied between the two extracts . CONCLUSIONS The unknown plant metabolites in both potato extracts were assigned with molecular formulae and identified with high confidence. Quantification of the metabolites was achieved using a number of appropriate standards. High‐resolution mass spectrometry data critical for accurate identification of unknown phytochemicals were achieved and could be added to potato or plant metabolomic database. © 2013 Society of Chemical Industry