Development of a rapid chemiluminescent ciELISA for simultaneous determination of florfenicol and its metabolite florfenicol amine in animal meat products

BACKGROUND A rapid one‐step chemiluminescent competitive indirect enzyme‐linked immunosorbent assay ( CL‐ciELISA ) for florfenicol ( FF ) and its major metabolite florfenicol amine ( FFA ) residues in animal meat products has been developed . RESULTS The 50% binding inhibition ( IC 50 ) values of the method were 0.195 µg kg −1 for FFA and 0.24 µg kg −1 for FF under optimum conditions. The cross‐reactive rates for FF and FFA were 100.0% and 81.2%, respectively. FF and FFA were easily extracted from animal meat product with an FF / FFA extraction buffer, obtaining recoveries of 81.8–92.0% ( FF ) and 77.2–100% ( FFA ). The whole one‐step CL‐ciELISA test can be accomplished within 40 min in theory. The detection limits ( LODs ) of the assay were 0.98 µg kg −1 for FF and 0.80 µg kg −1 for FFA in animal meat samples. Finally, field animal meat samples were analyzed with the CL‐ciELISA method, and the results correlated well with those obtained using traditional ELISA and a previously reported liquid chromatographic–tandem mass spectrometric method.CONCLUSION The combined results confirmed the utility of this faster one‐step CL‐ciELISA for simultaneous trace analysis of FF and FFA . To date, this is the most rapid developed ELISA and CL‐ELISA method for detection of FF and FFA . © 2013 Society of Chemical Industry