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Physicochemical properties and antimicrobial activity of Roselle ( Hibiscus sabdariffa L.)
Author(s) -
Jung EunKyung,
Kim YoungJun,
Joo Nami
Publication year - 2013
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.6256
Subject(s) - hibiscus sabdariffa , chemistry , antimicrobial , dpph , ascorbic acid , food science , antioxidant , staphylococcus aureus , extraction (chemistry) , chromatography , traditional medicine , biochemistry , bacteria , organic chemistry , biology , medicine , genetics
BACKGROUND The therapeutic action of a plant depends on its chemical constituents. In this study, experiments were carried out in order to evaluate the effect of extraction conditions on the antioxidative and antimicrobial activities of Roselle ( Hibiscus sabdariffa L.) . RESULTS Roselle was found to be rich in malic acid, anthocyanins, ascorbic acid and minerals, especially Ca and Fe, but low in glucose. More than 18 volatile compounds were identified by gas chromatography and gas chromatography–mass spectrometry. This herb, which is rich in phenolic compounds and displays DPPH radical scavenging activity, could be a good source of natural antioxidants. The antimicrobial activity of the Roselle water and ethanol extracts was tested with Bacillus subtilis ( ATCC6633 ), Staphylococcus aureus ( ATCC6538 ) and Escherichia coli ( ATCC 8739). The inhibition of the Roselle ethanol extract against B. subtilis and S. aureus was slightly higher than that of water extract but this difference was not significant. However, E. coli was strongly inhibited by the Roselle water extract at concentrations of 25 and 50 mg mL −1 as determined by a paper disc method . CONCLUSION The obtained results indicated that antioxidant and antimicrobial activity was related to different methods of extraction and Roselle extracts could be a source of therapeutically useful products. © 2013 Society of Chemical Industry