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Enhanced production of pigments by addition of surfactants in submerged fermentation of Monascus purpureus H1102
Author(s) -
Wang Yonghui,
Zhang Bobo,
Lu Liping,
Huang Yan,
Xu Ganrong
Publication year - 2013
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.6182
Subject(s) - monascus purpureus , monascus , pigment , fermentation , food science , pulmonary surfactant , chemistry , intracellular , fatty acid , extracellular , biochemistry , biology , organic chemistry
BACKGROUND The production of pigments by Monascus spp. has attracted increasing attention. Modification of the cell membrane structure by addition of surfactants has proved to be effective for the secretion of intracellular metabolites. Hence in this study the effects and underlying mechanism of surfactants on the production of pigments in submerged fermentation of Monascus purpureus H1102 were systematically investigated.RESULTS Various surfactants exerted significant but different impacts on the biomass and production of pigments. The maximum production of pigment (304.3 U mL −1 ) and highest extracellular/intracellular pigment ratio (1.46) were achieved when 15 g L −1 Triton X‐100 was added at 24 h of fermentation, corresponding to significant increases of 88.4 and 240% respectively compared with the control. Meanwhile, the concentration of citrinin (0.94 mg L −1 ) was 20.6% lower than that of the control. A further study on the fatty acid composition of M. purpureus H1102 showed that the unsaturated/saturated fatty acid ratio and the index of unsaturated fatty acid increased significantly with the addition of Triton X‐100.CONCLUSION The addition of surfactant Triton X‐100 could greatly enhance the production of pigment. It was suggested that Triton X‐100 facilitated the secretion of intracellular pigment and therefore enhanced pigment production accordingly.© 2013 Society of Chemical Industry