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Determination of the variations in levels of phenolic compounds in soybean ( Glycine max Merr.) sprouts infected by anthracnose ( Colletotrichum gloeosporioides )
Author(s) -
Lee Jung Han,
Jeong Sung Woo,
Cho Young Ah,
Park Semin,
Kim Yun-Hi,
Bae Dong Won,
Chung Jong Il,
Kwak YounSig,
Jeong MiJeong,
Park SooChul,
Shim JaeHan,
Jin Jong Sung,
Shin Sung Chul
Publication year - 2013
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.6142
Subject(s) - daidzein , genistin , daidzin , glycitein , coumestrol , glycine , isoflavones , genistein , biology , food science , inoculation , chemistry , botany , horticulture , biochemistry , amino acid , endocrinology
Abstract BACKGROUND Soybean sprouts (Kongnamool) are one of the most popular and nutritive traditional vegetables in East Asia. Anthracnose caused by Colletotrichum gloeosporioides is one of the most serious diseases of soybean sprouts. In order to obtain basic information for breeding and/or selecting soybean genotypes with increased natural defense against anthracnose, phenolic compounds were profiled for healthy and infected soybean ( Glycine max Merr.) sprouts by using high‐performance liquid chromatography coupled with tandem mass spectrometry . RESULTS Tryptophan and eight phenolic compounds (daidzin, genistin, malonyldaidzin, malonylgenistin, daidzein, glycitein, genistein and coumestrol) were determined from healthy and inoculated sprouts. Total identified phenolic content was 40.02 ± 0.03 mg kg −1 , 99.4% of which was isoflavones . CONCLUSION The monitoring suggested that de novo induced glycitein appeared to act as a phytoalexin in the defence mechanism of the soybean sprouts against C. gloeosporioides , and constitutively formed seven phenolic components that functioned as phytoanticipins in the diseased soybean sprouts . © 2013 Society of Chemical Industry