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Phylogenetic analysis, fumonisin production and pathogenicity of Fusarium fujikuroi strains isolated from rice in the Philippines
Author(s) -
Cruz Alejandra,
Marín Patricia,
GonzálezJaén M Teresa,
Aguilar Kristel Grace I,
Cumagun Christian Joseph R
Publication year - 2013
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.6136
Subject(s) - fumonisin , biology , fusarium proliferatum , fusarium , phylogenetic tree , mycotoxin , phylogenetics , gene , genbank , fumonisin b1 , microbiology and biotechnology , botany , genetics
BACKGROUND Fusarium fujikuroi Nirenberg is a maize and rice pathogen causing important agricultural losses and produces fumonisins – mycotoxins which pose health risk to humans and farm animals. However, little information is available about the phylogenetics of this species and its ability to produce fumonisins in rice. We studied 32 strains isolated from rice in the Philippines and performed a phylogenetic analysis using the partial sequence of Elongation Factor 1 alpha ( EF ‐1α) including isolates belonging to closely related species. Fumonisin B 1 ( FB 1 ) production was analyzed in 7‐day‐old cultures grown in fumonisin‐inducing medium by an enzyme‐linked immunosorbent assay‐based method and by real‐time reverse transcriptase–polymerase chain reaction using primers for FUM1 gene, a key gene in fumonisin biosynthesis.RESULTS Nucleotide diversities per site ( π ) were 0.00024 ± 0.00022 (standard deviation) for the 32 F. fujikuroi strains from the Philippines and 0.00189 ± 0.00143 for all 34 F. fujikuroi strains, respectively. F. fujikuroi isolates grouped into one cluster separated from the rest of isolates belonging to the closely related F. proliferatum and showed very low variability, irrespective of their geographic origin. The cluster containing strains of F. proliferatum showed higher intraspecific variability than F. fujikuroi . Thirteen of the 32 strains analyzed were FB 1 producers (40.62%), with production ranging from 0.386 to 223.83 ppm. All isolates analyzed showed FUM1 gene expression above 1 and higher than the C T value of the non‐template control sample. Both seedling stunting and elongation were induced by the isolates in comparison with the control.CONCLUSION F. fujikuroi are distinct from F. proliferatum isolates based on phytogenetic analysis and are potential fumonisin producers because all are positive for FUM1 gene expression. No relationship between fumonisin production and pathogenicity could be observed. © 2013 Society of Chemical Industry