Premium
Inhibitory effect of unicellular green algae ( Chlorella vulgaris ) water extract on allergic immune response
Author(s) -
Bae MinJung,
Shin Hee Soon,
Chai Ok Hee,
Han JaeGab,
Shon DongHwa
Publication year - 2013
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.6114
Subject(s) - histamine , immunoglobulin e , immune system , splenocyte , ovalbumin , allergic response , degranulation , chemistry , cytokine , antihistamine , secretion , immunology , biology , pharmacology , antibody , biochemistry , receptor
Background Chlorella is used as a functional food in East Asia and has been shown to enhance immune system function. However, there has been no direct evidence of the suppressive effect of a hot water extract of Chlorella vulgaris ( CVE ) on histamine‐mediated allergic responses . Results The antihistamine activity of CVE was analysed using rat peritoneal mast cells ( RPMCs ) stimulated by compound 48/80. For in vivo verification, ovalbumin ( OVA )‐immunised BALB /c mice were treated with CVE orally. Serum immunoglobulin E ( IgE ) levels and splenocyte cytokine levels were determined by enzyme‐linked immunosorbent assay ( ELISA ). CVE prevented histamine release through degranulation of mast cells by blocking the uptake of extracellular Ca 2+ into RPMCs . Moreover, CVE administration inhibited serum IgE overproduction by OVA via induction of T helper 1 (Th1) skewing that was dependent on interferon‐ γ ( IFN ‐ γ ) and interleukin 12 ( IL ‐12) secretion . Conclusion The results of this study clearly demonstrate that CVE acts as an antiallergic dietary agent by suppressing histamine release via its enhancive effect on Th1‐related responses. © 2013 Society of Chemical Industry