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Barcode DNA high‐resolution melting (Bar‐ HRM ) analysis as a novel close‐tubed and accurate tool for olive oil forensic use
Author(s) -
Ganopoulos Ioannis,
Bazakos Christos,
Madesis Panagiotis,
Kalaitzis Panagiotis,
Tsaftaris Athanasios
Publication year - 2013
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.6040
Subject(s) - canola , olive oil , barcode , vegetable oil , dna barcoding , high resolution melt , food science , traceability , high resolution , edible oil , environmental science , pulp and paper industry , biology , computer science , engineering , geology , polymerase chain reaction , remote sensing , ecology , biochemistry , software engineering , gene , operating system
Abstract BACKGROUND The adulteration of high‐priced olive oil with low‐cost oils and the fraudulent labelling of oil products make the identification and traceability of vegetable oil species in the food chain very important. This paper describes a high‐resolution melting analysis‐based method using chloroplast barcoding regions as target (Bar‐ HRM ) to obtain barcoding information for the major vegetable oil species and to quantitatively identify the botanical origin of plant oils. The detection of adulteration of olive oil with canola oil was used as a case study.RESULTS The proposed method was capable of distinguishing among different vegetable oil species and detecting a level of 1% (w/w) of canola oil in olive oil.CONCLUSION Bar‐ HRM analysis is a more accurate, faster and less costly alternative method to authenticate vegetable oils, including olive oil, and to detect mixtures of oils. © 2013 Society of Chemical Industry