Premium
Control of Phytophthora nicotianae disease, induction of defense responses and genes expression of papaya fruits treated with Pseudomonas putida MGP1
Author(s) -
Shi Jingying,
Liu Aiyuan,
Li Xueping,
Chen Weixin
Publication year - 2013
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.5831
Subject(s) - phenylalanine ammonia lyase , chitinase , biology , phytophthora nicotianae , pseudomonas putida , peroxidase , polyphenol oxidase , microbiology and biotechnology , carica , catalase , plant disease resistance , gene , botany , horticulture , phytophthora , enzyme , biochemistry
BACKGROUND: Biological control is a potential strategy to reduce post‐harvest decay in several fruits. Little research has been carried out on the effects of endophytic bacterium on post‐harvest blight caused by Phytophthora nicotianae in papaya. In this work, the biocontrol activity of Pseudomonas putida MGP1 on this disease and its possible mechanisms, including changes of defensive enzyme activities, total phenolic content and mRNA levels of two important genes, were investigated. RESULTS: Fruits treated with MGP1 showed a significant lower disease index and demonstrated increases in chitinase, β‐1,3‐glucanase, phenylalanine ammonia‐lyase, peroxidase, polyphenol oxidase and catalase activities and total phenolic content. In addition, the expression levels of pathogenesis related protein 1 gene ( PR1 ) and non‐expressor of PR1 gene ( NPR1 ) in papaya fruits were elevated by MGP1 treatment. CONCLUSION The results indicated that papaya fruits were responsive to the endophytic bacterium Ps. putida , which could activate defensive enzymes and genes and thereby induce host disease resistance. © 2012 Society of Chemical Industry