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In vitro and in vivo antifungal activity of a water‐dilutable cassia oil microemulsion against Geotrichum citri‐aurantii
Author(s) -
Xu ShiXiang,
Li YiChen,
Liu Xia,
Mao LiJuan,
Zhang Hui,
Zheng XiaoDong
Publication year - 2012
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.5686
Subject(s) - microemulsion , postharvest , cassia , chemistry , in vivo , pulmonary surfactant , chromatography , food science , biology , botany , microbiology and biotechnology , biochemistry , medicine , alternative medicine , traditional chinese medicine , pathology
BACKGROUND: Recently, food‐grade microemulsions have been of increasing interest to researchers and have shown great potential in industrial applications. In this study a food‐grade water‐dilutable microemulsion system with cassia oil as oil, ethanol as cosurfactant, Tween 20 as surfactant and water was developed and its antifungal activity in vitro and in vivo against Geotrichum citri‐aurantii was assessed. RESULTS: The phase diagram results confirmed the feasibility of forming a water‐dilutable microemulsion based on cassia oil. One microemulsion formulation, cassia oil/ethanol/Tween 20 = 1:3:6 (w/w/w), was selected with the capability to undergo full dilution with water. The average particle size was 6.3 nm. The in vitro antifungal experiments showed that the microemulsion inhibited fungal growth on solid medium and prevented arthroconidium germination in liquid medium and that cassia oil had stronger activity when encapsulated in the microemulsion. The in vivo antifungal experiments indicated that the water‐dilutable microemulsion was effective in preventing postharvest diseases of citrus fruits caused by G. citri‐aurantii . CONCLUSION: The results of this study suggest a promising utilisation of water‐dilutable microemulsions based on essential oils for the control of postharvest diseases. Copyright © 2012 Society of Chemical Industry