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Nepeta japonica Maximowicz extract from natural products inhibits lipid accumulation
Author(s) -
Roh Changhyun,
Jung Uhee
Publication year - 2012
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.5608
Subject(s) - lipase , triglyceride , japonica , chemistry , obesity , biochemistry , pharmacology , food science , biology , enzyme , cholesterol , botany , endocrinology
BACKGROUND: To develop novel and crude anti‐obesity drugs from natural products is a promising field to approach the solution to a global health problem such as obesity. The aim of this study was to screen crude anti‐obesity drugs from 400 natural products on lipase inhibition activity in vitro . RESULTS: Among the natural products examined, 31 extracts showed significantly inhibition activity against porcine pancreatic lipase (triacylglycerol lipase, EC 3.1.1.3) by using spectrophotometry with 2,4‐dinitrophenylbutyrate as a substrate. Furthermore, 31 natural products were investigated with regard to their lipid inhibition in 3T3‐L1 cells. Among these, one of most promising was Nepeta japonica Maximowicz extract, which showed inhibition of triglyceride accumulation in 3T3‐L1 adipocytes, suggesting anti‐obesity activity. Also, the amount of glycerol released from cells into the medium was increased by treatment of Nepeta japonica Maximowicz extract at a concentration of 100 µg mL −1 . CONCLUSION: The present study suggests that a promising crude anti‐obesity drug screened from 400 natural products might be of therapeutic interest with respect to the treatment of obesity. Copyright © 2012 Society of Chemical Industry