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Isolation and anti‐inflammatory effect of astragalin synthesized by enzymatic hydrolysis of tea seed extract
Author(s) -
Lee HyangBok,
Kim EunKi,
Park SangJae,
Bang Sanggu,
Kim Tae Gil,
Chung Daewon
Publication year - 2011
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.4457
Subject(s) - astragalin , chemistry , enzymatic hydrolysis , hydrolysis , food science , kaempferol , nitric oxide , enzyme , flavonoid , biochemistry , organic chemistry , antioxidant
BACKGROUND: The application of tea seed extract (TSE) has been widely investigated because of its biological activities. In this paper, two flavonol triglycosides in TSE—camelliaside A (CamA) and camelliaside B (CamB)—were subjected to hydrolysis in the presence of two commercial enzyme complexes (Pectinex ™ series): Smash and Mash. RESULTS: Smash hydrolyzed only the xylosyl moiety of CamB, and the main product was kaempferol diglycoside (nicotiflorin, NF). On the other hand, Mash induced the hydrolysis of both CamA and CamB, and kaempferol monoglycoside (astragalin, AS) was found to be a main product. Pure AS with > 96% purity was prepared by enzymatic hydrolysis of TSE using Mash, and the chemical structure of AS was confirmed by 1 H‐ and 13 C‐nuclear magnetic resonance analyses. The prepared pure AS showed anti‐inflammatory activities by significantly inhibiting cellular nitrite oxide (IC 50 = 363 µg mL −1 ), prostaglandin E 2 (IC 50 = 134 µg mL −1 ) and interleukin‐6 production (IC 50 = 289 µg mL −1 ) by lipopolysaccharide ‐stimulated RAW 264.7 cells. CONCLUSION: It was concluded that pure AS can be prepared by enzymatic partial hydrolysis of TSE and employed as an anti‐inflammatory material. This is the first study to address the preparation of pure AS from natural sources. Copyright © 2011 Society of Chemical Industry