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Evaluation of ISSR markers for genetic diversity analysis in Anatolian Water Buffaloes
Author(s) -
Aytekin Ibrahim,
Ozdil Fulya,
Zulkadir Ugur,
Boztepe Saim,
Sariyel Vahdettin
Publication year - 2011
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.4397
Subject(s) - genetic diversity , loss of heterozygosity , biology , microsatellite , genetics , evolutionary biology , veterinary medicine , allele , gene , population , demography , medicine , sociology
BACKGROUND: The utilisation of molecular markers has increased in molecular research recently. Inter‐simple sequence repeat (ISSR) markers allow the analysis of genomes without preliminary sequence information, since random primers are used. The purpose of the present study was to evaluate ISSR markers for assessing the genetic diversity of indigenous Anatolian Water Buffaloes reared in Afyon, Konya and Sivas provinces of Turkey, with a view to conservation of the gene resources. RESULTS: The 11 ISSR primers chosen for the analysis revealed a total of 110 bands, of which 76 (69.09%) were polymorphic. Also, genetic similarity, polymorphic information content (PIC), resolving power ( R p ) and mean resolving power ( R p ), heterozygosity ( H ) and Shannon index ( I ) were calculated as 0.9479–0.9562, 0.35 ± 0.20, 2.73, 0.27, 0.18 ± 0.07 and 0.28 ± 0.11, respectively. CONCLUSION: The ISSR markers were found to be promising for assessing the genetic diversity in buffalo populations. Potential genetic parameters such as PIC, R p , R p , H and I were effectively used in this study. Copyright © 2011 Society of Chemical Industry