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The impact of transgenic papaya (TPY10‐4) fruit supplementation on immune responses in ovalbumin‐sensitised mice
Author(s) -
Chen YiNing,
Hwang WenZhe,
Fang Tony J,
Cheng Ying Huey,
Lin JinYuarn
Publication year - 2011
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.4218
Subject(s) - ovalbumin , immunoglobulin e , antibody , splenocyte , biology , immune system , immunology
BACKGROUND: A transgenic papaya line (TPY10‐4) that is resistant to both papaya ringspot virus (PRSV) and papaya leaf distortion mosaic virus (PLDMV) has been developed in Taiwan. This study investigated the immunomodulatory properties of transgenic TPY10‐4 and its native (TCK) papaya fruits using an ovalbumin (OVA)‐sensitised mouse model. Both green and ripe papaya fruits at low (0.2 g powder kg −1 body weight (BW)) and high (1.6 g powder kg −1 BW) doses were administered to experimental mice by intragastric gavage for 5 weeks. Changes in serum total immunoglobulin A (IgA), IgE, IgG and IgM levels, OVA‐specific IgE, IgG1 and IgG2a titres and Th1/Th2 cytokine secretions using splenocytes were determined. RESULTS: Transgenic TPY10‐4 or native TCK papaya fruit supplementation did not significantly affect body, visceral organ and relative tissue weights, total IgE antibody levels, OVA‐specific IgE and IgG1 antibody titres or OVA‐stimulated interferon‐γ (IFN‐γ), interleukin‐2 (IL‐2), IL‐4, IL‐5 and IL‐10 secretions using splenocytes. However, transgenic papaya fruits markedly increased serum total IgM levels. CONCLUSION: This study suggests that transgenic TPY10‐4 papaya fruits do not increase the allergenic potential of OVA by oral administration but may have a protective immunity via increasing the serum total IgM level. Copyright © 2010 Society of Chemical Industry