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Vascular effects of egg white‐derived peptides in resistance arteries from rats. Structure–activity relationships
Author(s) -
GarciaRedondo Ana B,
Roque Fernanda R,
Miguel Marta,
LópezFandiño Rosina,
Salaices Mercedes
Publication year - 2010
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.4037
Subject(s) - vasodilation , myograph , chemistry , nitric oxide , mesenteric arteries , peptide , endocrinology , medicine , vasoconstriction , endothelium , biochemistry , pharmacology , artery
Abstract BACKGROUND: The vasodilator properties of several peptide sequences derived from egg white proteins were screened in mesenteric resistance arteries from Wistar‐Kyoto rats. For this, third‐order branches of the mesenteric arteries from 6‐month‐old male rats were used. The vasodilator responses, with or without endothelium, to several peptides (0.1 mmol L −1 ) were analysed in an isometric myograph. Moreover, the effect of nitric oxide (NO) synthase (L‐NAME, 100 µmol L −1 ) and cyclooxygenase (indomethacin, 10 µmol L −1 ) inhibitors on the vasodilator response was tested. RESULTS: The peptides Arg‐Ala‐Asp‐His‐Pro‐Phe‐Leu, Arg‐Ala‐Asp‐His‐Pro‐Phe, Arg‐Ala‐Asp‐His‐Pro, Tyr‐Arg‐Gly‐Gly‐Leu‐Glu‐Pro‐Ile‐Asn‐Phe, Arg‐Asp‐Ile‐Leu‐Asn‐Gln and Val‐Pro‐Pro showed a high endothelium‐dependent vasorelaxation, whereas Phe‐Arg‐Ala‐Asp‐His‐Pro‐Phe‐Leu was only partially endothelium‐dependent. The relaxation induced by Arg‐Ala‐Asp‐His‐Pro‐Phe‐Leu, Arg‐Ala‐Asp‐His‐Pro‐Phe, Arg‐Ala‐Asp‐His‐Pro, Arg‐Asp‐Ile‐Leu‐Asn‐Gln and Val‐Pro‐Pro was mainly mediated by NO, since the response was inhibited only by L‐NAME, while both L‐NAME and indomethacin inhibited the vasodilator response induced by Phe‐Arg‐Ala‐Asp‐His‐Pro‐Phe‐Leu and Tyr‐Arg‐Gly‐Gly‐Leu‐Glu‐Pro‐Ile‐Asn‐Phe. The presence of Arg or Tyr at the N‐terminal position could be related to the vasodilator activity of these compounds in this vascular bed. The well‐known angiotensin‐converting enzyme inhibitor captopril showed only a slight vasodilator effect. CONCLUSION: These peptides could reduce the vascular resistance and be used as functional ingredients in the prevention and/or treatment of hypertension and other associated disorders. Copyright © 2010 Society of Chemical Industry