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Identification of repressed gene transcript accumulation in three albino mutants of Bambusa edulis Munro by cDNA microarray analysis
Author(s) -
Wu FuHui,
Liu NienTze,
Chou ShuJen,
Shen ShuChen,
Chang Bill Chiahan,
Pan ChaoYu,
Lin ChounSea
Publication year - 2009
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.3725
Subject(s) - mutant , biology , complementary dna , gene , microarray analysis techniques , microbiology and biotechnology , gene expression , gene isoform , microarray , wild type , genetics
BACKGROUND: Bamboo is an economically important market vegetable and processed food product in Asia. An accelerated growth rate and increased photosynthesis efficiency would be valuable agronomic traits in commercial bamboo culture. Tissue culture‐derived albino mutants can be used as experimental material to investigate this phenomenon, but the current body of genetic information available on bamboo is very limited. RESULTS: A cDNA library was constructed from in vitro shoots of Bambusa eduli s Munro and subsequently used in a bamboo microarray analysis to investigate the gene profiles of three albino mutants ( ab, ab1, ab2 ) cultured under light/dark and dark conditions. Analysis of the array data suggested that no gene transcript accumulation was significantly upregulated, but that transcript accumulation of 24 genes was downregulated by <50% relative to the wild type. This repression was determined to be light‐independent as these genes were also repressed when ab mutant shoots were incubated in the dark. Microarray analysis of the light sensitivity of specific gene expression of the mutant ab and wild type shoots identified 14 genes as being highly expressed when cultured in the light, 12 of which showed a similar response to light in both the wild type and ab mutant. The transcripts of a group of bamboo‐specific genes (three isoforms) accumulated less in all albino mutants, and another group of bamboo‐specific genes (six isoforms) accumulated less under dark conditions. The microarray results were confirmed by reverse transcriptase polymerase chain reaction. Using the rice protoplast transformation, the products of the bamboo‐specific albino‐repressed gene 09A06 were found to be localized to the chloroplast. CONCLUSION: A Bambusa edulis shoot cDNA library was used to establish a bamboo microarray, which successfully identified gene transcripts that were repressed in the albino mutants. The subcellular localization of these bamboo‐specific gene products was identified by rice protoplast transient expression, where 09A06 accumulated around the chloroplast. Copyright © 2009 Society of Chemical Industry