Premium
Effect of long‐term dietary administration of oregano and rosemary on the antioxidant status of rat serum, liver, kidney and heart after carbon tetrachloride‐induced oxidative stress
Author(s) -
Botsoglou Nikolas A,
Taitzoglou Ioannis A,
Botsoglou Evropi,
Zervos Ioannis,
Kokoli Alexandra,
Christaki Efterpi,
Nikolaidis Efstathios
Publication year - 2009
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.3601
Subject(s) - chemistry , lipid peroxidation , carbon tetrachloride , antioxidant , oxidative stress , hydroxyl radical , aspartate transaminase , superoxide , hydrogen peroxide , alanine transaminase , alkaline phosphatase , abts , lipid peroxide , medicine , endocrinology , biochemistry , food science , dpph , enzyme , biology , organic chemistry
BACKGROUND: This study was performed on female Wistar rats allocated to eight groups of six animals each. Groups 1 and 2 were fed the basal diet, groups 3 and 4 were fed the basal diet supplemented with ground oregano at 20 g kg −1 level, groups 5 and 6 were fed the basal diet supplemented with ground rosemary at 20 g kg −1 level, while groups 7 and 8 were fed the basal diet supplemented with both oregano and rosemary, each at 20 g kg −1 level. Following 6 weeks feeding, groups 2, 4, 6 and 8 were injected with CCl 4 at 1 mL kg −1 body weight, and 6 h thereafter all animals were sacrificed. RESULTS: Administration of CCl 4 to the control rats enhanced ( P < 0.05) aspartate transaminase (AST), alanine transaminase (ALT) and alkaline phosphatase (ALP) activities, decreased cholesterol and triglycerides content in serum, increased ( P < 0.05) lipid peroxidation and decreased ( P < 0.05) the ABTS radical cation, the hydroxyl anion radical, the superoxide anion radical, and the hydrogen peroxide scavenging activities in all tested tissues, as compared to the control. Feeding oregano, rosemary or both before CCl 4 treatment resulted in decline ( P < 0.05) of the increase in AST, ALT and ALP activities, increase ( P < 0.05) of cholesterol and triglycerides in serum, decrease ( P < 0.05) of lipid peroxidation, and increase ( P < 0.05) of the ABTS radical cation, hydroxyl anion radical, superoxide anion radical and hydrogen peroxide scavenging activity. CONCLUSION: The results of this study suggest that long‐term dietary administration of oregano and rosemary has the potential to quench free radicals and alleviate CCl 4 ‐induced oxidative stress in rats. Copyright © 2009 Society of Chemical Industry