Premium
Expression analysis of endo‐1,4‐β‐glucanase genes during aril breakdown of harvested longan fruit
Author(s) -
Xiao Ruo,
Chen Jianye,
Chen Jianwen,
Ou Min,
Jiang Yueming,
Lin Hetong,
Lu Wangjin
Publication year - 2009
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.3562
Subject(s) - aril , chemistry , horticulture , botany , food science , biology
BACKGROUND: The aim of the present study was to investigate the expression profiles of three endo‐1,4‐β‐glucanase (EGase, EC 3.2.1.4) genes during aril breakdown of longan fruit stored at room temperature (25 °C), low temperature (10 °C) or on transferring fruit stored at 10 °C for 20 days to 25 °C. RESULTS: Three longan full‐length cDNAs, designated Dl‐EGase1, Dl‐EGase2 and Dl‐EGase3 , were isolated and characterized. EGase activity in aril tissues of longan fruit increased with the appearance of aril breakdown symptoms, while RNA gel blot analysis revealed that the accumulations of three Dl‐EGase genes exhibited differential characteristics with the occurrence of aril breakdown. Dl‐EGase2 may be involved in the aril breakdown in longan fruit at the later stage of storage at room temperature. Conversely, expression of Dl‐EGase3 could be mainly involved in aril breakdown of fruit stored at 10 °C. In addition, Dl‐EGase3 and Dl‐Egase2 were related to the aril breakdown of fruit transferred from low temperature to room temperature. CONCLUSION: The results obtained in this study indicate that Dl‐EGase genes are involved in the aril breakdown of longan fruit and that considerable variation exists between expression patterns of individual members of the EGase gene family. Copyright © 2009 Society of Chemical Industry