Premium
Densitometric determination of zinc bacitracin and nystatin in animal feed
Author(s) -
Injac Rade,
Strukelj Borut,
DjordjevicMilic Vukosava,
KarljikovicRajic Katarina,
Lingeman Henk
Publication year - 2008
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.3253
Subject(s) - bacitracin , chemistry , chromatography , zinc , feed additive , methanol , european union , nystatin , toluene , antibiotics , food science , biochemistry , broiler , organic chemistry , business , economic policy
BACKGROUND: The European Union has forbidden the use of antibiotics as additives in animal feed. Zn‐bacitracin (Zn‐BC) and nystatin (NYS) were frequently used for their growth‐promoting effects and for feed conversion in poultry, pigs and cattle. An HPTLC method has been developed for separating Zn‐BC and NYS in the mixture, for routine quality control. RESULTS: The separation was obtained using RP‐18 F 254S coated HPTLC plates with acetonitrile/methanol (equal volumes):toluene:KH 2 PO 4 /KOH (buffer, pH 6.8) = 57:3:40 (v/v/v), adjusted with HCl to pH 8.2, as a mobile phase. The densitograms were monitored at 192, 215 and 305 nm and both antibiotics were assayed at 215 nm. The method was shown to be specific, accurate (recoveries were 98.7 ± 0.5% and 104.8 ± 0.7% for Zn‐BC and NYS, respectively), linear over the tested range (correlation coefficients, 0.9982 and 0.9884), and precise (intermediate precision RSD below 2.2% for both analytes) with efficient separation ( R s = 3.5). CONCLUSION: The method was applied for determining Zn‐BC and NYS as additives in spiked matrices of commercial animal feedstuffs. According to LOD values for each antibiotic, the minimum detectable amount in feed is 4.5 and 5.5 ppm of Zn‐BC and NYS, respectively. Copyright © 2008 Society of Chemical Industry