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Flavonoid content and antioxidant capacity of spinach genotypes determined by high‐performance liquid chromatography/mass spectrometry
Author(s) -
Cho Mi Jin,
Howard Luke R,
Prior Ronald L,
Morelock Teddy
Publication year - 2008
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.3206
Subject(s) - oxygen radical absorbance capacity , chemistry , flavonols , flavonoid , chromatography , antioxidant , mass spectrometry , spinach , high performance liquid chromatography , food science , antioxidant capacity , biochemistry
BACKGROUND: Flavonoids in different spinach genotypes were separated, identified, and quantified by a high‐performance liquid chromatographic method with photodiode array and mass spectrometric detection. The antioxidant capacities of the genotypes were also measured using two antioxidant assays–oxygen radical absorbance capacity (ORAC FL ) and photochemiluminescence (PCL)–which measure the response to the peroxyl and superoxide anion radicals, respectively. RESULTS: Eighteen flavonoids representing glucuronides and acylated di‐ and triglycosides of methylated and methylene dioxide derivatives of 6‐oxygenated flavonols were identified (patuletin, spinacetin, spinatoside, jaceidin). The total flavonoids ranged from 1805 to 3703 mg kg −1 , indicating 2.0‐fold variation among genotypes. The ORAC FL and PCL values ranged from 48.7 to 84.4 mmol kg −1 and from 9.0 to 14.0 mmol kg −1 , respectively, representing as much as 1.7‐fold variation among genotypes. CONCLUSION: The ORAC FL and PCL values were highly correlated with total flavonoid content ( r xy = 0.96). Copyright © 2008 Society of Chemical Industry