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Proteomic analysis of the distribution of the major seed allergens in wild, landrace, ancestral, and modern soybean genotypes
Author(s) -
Xu Chenping,
Caperna Thomas J,
Garrett Wesley M,
Cregan Perry,
Bae Hanhong,
Luthria Devanand L,
Natarajan Savithiry
Publication year - 2007
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.3017
Subject(s) - genotype , spots , biology , storage protein , protein subunit , veterinary medicine , horticulture , botany , genetics , gene , medicine
BACKGROUND: A comparative analysis of seed allergens from various soybean genotypes is crucial for identifying and eliminating potential allergens. We have investigated the distribution of three major allergens (Gly m Bd 60K, Gly m Bd 30K and Gly m Bd 28K) in wild, landrace, ancestral and modern soybean genotypes. RESULTS: Gly m Bd 60K allergens consist of α subunits of β‐conglycinin and G2 subunits of glycinin. In wild genotypes, α subunits of β‐conglycinin separated into six to seven protein spots whereas five to seven spots were observed in the landraces. All genotypes of modern and ancestral groups showed 3–5 protein spots of α subunits of β‐conglycinin. All genotypes showed eight spots of glycinin G2 subunits except one ancestral genotype which had seven spots. Two protein spots were detected for Gly m Bd 30K in 14 genotypes but one spot was detected in two wild genotypes. Two protein spots were detected for Gly m Bd 28K in all genotypes. CONCLUSION: Considerable heterogeneity of the α subunit of β‐conglycinin distribution exists among these 16 soybean genotypes. Significant proteomic variation was observed between different soybean groups rather than among genotypes in the same group. This investigation would be valuable to researchers working with soybean and nutrition. Copyright © 2007 Society of Chemical Industry