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In‐house validation of a high‐performance liquid chromatography analytical method for quantification of ochratoxin A in unfermented grape juice
Author(s) -
Murillo Ma Teresa,
GonzálezPeñas Elena,
Amézqueta Susana,
de Cerain Adela López
Publication year - 2007
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2980
Subject(s) - chromatography , ochratoxin a , chemistry , detection limit , high performance liquid chromatography , food science , mycotoxin
A validated high‐performance liquid chromatography (HPLC) method with fluorescence detection for the quantitative analysis of ochratoxin A (OTA) in unfermented grape juice is described. Five millilitres of unfermented grape juice was mixed with 45 mL of PBS, and the pH was adjusted to 7.2. Then the mixture was filtered under vacuum through a glass microfibre filter and cleaned up with immunoaffinity columns prior to analysis by HPLC. Validation of the analytical method was based on the following criteria: selectivity, linearity, limit of detection and quantification, precision (within‐day and between‐day variability) and recovery and uncertainty estimation. Detection and quantification limits obtained were 0.02 µg L −1 and 0.05 µg L −1 respectively. The percentage recovery was 91.5% ( RSD = 3.9). This method was applied to the measurement of 30 veraison stage unfermented grape juice samples. Copyright © 2007 Society of Chemical Industry