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Identification of Tuber spp and corresponding ectomycorrhizae through molecular markers
Author(s) -
Paolocci Francesco,
Angelini Paola,
Cristofari Ettore,
Granetti Bruno,
Arcioni Sergio
Publication year - 1995
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740690416
Subject(s) - ectomycorrhizae , biology , genomic dna , primer (cosmetics) , botany , polymerase chain reaction , ribosomal dna , fungus , host (biology) , internal transcribed spacer , mycorrhiza , ribosomal rna , dna , symbiosis , bacteria , phylogenetics , gene , genetics , chemistry , organic chemistry
Analyses, based on the structure of the rDNA region, were developed to examine the possibility of identifying and typing some of the most important Tuber spp at both the symbiotic and non‐symbiotic levels. Internal transcribed spacers (ITS) were amplified by the polymerase chain reaction using specific primer pairs on genomic DNAs, isolated from ascocarps and ectomycorrhizae of various Tuber spp and from rootlets of uninoculated host plants. The variability in the lengths of ITS amplification products of DNA isolated from fruit bodies allowed a primary discrimination of the Tuber spp considered. At the ecto‐mycorrhizal level the same species showed a pattern which was the combination of those typical of Tuber plus the host plants. Southern analyses, performed using the ITS product of Tuber brumale as a probe, confirmed the previous results: each fungus species showed a specific pattern, as well as each Tuber ‐host plant combination. It is concluded that molecular markers from the rDNA region can be used to differentiate Tuber spp and that the method proposed could be used to check and certify mycorrhizally infected plants.