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Studies on the effect of protectants on Lactobacillus acidophilus strain dehydrated under controlled low‐temperature vacuum dehydration and freeze‐drying by using response surface methodology
Author(s) -
King V AnErl,
Lin HaurJie
Publication year - 1995
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740680209
Subject(s) - dehydration , liter , glycerol , response surface methodology , chemistry , freeze drying , lactobacillus acidophilus , maximum temperature , chromatography , biochemistry , biology , thermodynamics , genetics , physics , probiotic , bacteria , endocrinology
Ca 2+ (0–60 g litre −1 ), glycerol (0–100 g litre −1 ), and non‐fat dry milk solids (NFDMS, 0–140 g litre −1 ) were combined to study their effects on the survival of Lactobacillus acidophilus in controlled low‐temperature vacuum dehydration and freeze‐drying. A three‐variable (each at three‐levels) design method was used and analysed by fitting a quadratic response surface. These factors were found to be significantly effective on survival. Fitted quadratic regression equations were plotted using a graphing software package. Conditions for the best survival of freeze‐drying was found to be Ca 2+ 5.34 g litre −1 . NFDMS 100.8 g litre −1 , and glycerol 41.5 g litre −1 and that of controlled low‐temperature vacuum dehydration was Ca 2+ 5.67 g litre −1 , NFDMS 107.1 g litre −1 , and glycerol 41.0 g litre −1 . Models obtained were tested using t ‐tests. Survival of freezing under different conditions was found to be not significantly different.