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In vivo breakdown of dietary [ methyl −14 C] and [uronate‐6 −14 C]pectin‐labelled spinach cell walls by rat intestinal microorganisms and incorporation of 14 C into host tissues
Author(s) -
Buchanan Callum J,
Fry Stephen C,
Eastwood Martin A
Publication year - 1995
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740680207
Subject(s) - pectin , spinach , cell wall , chemistry , caecum , in vivo , metabolism , polysaccharide , biochemistry , methanol , chromatography , organic chemistry , biology , medicine , microbiology and biotechnology
The metabolism of dietary plant cell wall (PCW) pectin was followed in the rat using PCW isolated from spinach cell cultures 14 C‐labelled in the galacturonic acid residues or pectic methyl ester groups. 14 C‐PCW were rapidly broken down in the caecum and colon of the rat and generated several groups of soluble products. [ 14 C]Methanol was released from [ methyl −14 C]pectin‐labelled PCW and some of the methanol was converted to 14 C‐labelled volatile acids. 14 C‐Labelled volatile acids were also generated from [ uronate ‐6‐ 14 C]pectin‐labelled PCW along with soluble but non‐volatile material. 14 C derived from both cell wall types was taken up by the caecal and colonic mucosa and transported to the liver, pelt and other body tissues. Some 14 C was excreted as 14 CO 2 and in urine.