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Metabolism of dietary ( Acetyl ‐1‐ 14 c)‐plant cell walls in the rat intestine
Author(s) -
Buchanan Callum J,
Fry Stephen C,
Eastwood Martin A
Publication year - 1995
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740670314
Subject(s) - spinach , caecum , biochemistry , urine , chemistry , metabolism , small intestine , large intestine , cell , biology , medicine
To determine a fate of acetyl groups in intact dietary plant cell walls (PCW) spinach cells were labelled using [1‐ 14 C]acetate as a precursor and the PCW isolated. The ( acetyl ‐ 14 C)‐PCW were fed to rats by garage and the 14 C traced in the rat gut and in the body tissues and excreta of the rat. Some label was solubilised during passage through the upper intestine of the rat, and subsequently incorporated into the body tissues of the rat. A small amount of the radioactivity was excreted as CO 2 (4%) and in urine (<1%). ( Acetyl ‐ 14 C)‐PCW which reached the caecum was solubilised and some [ 14 C]acetate released. The solubilised 14 C was incorporated in liver tissue mainly as amino acid present in protein.