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Lectins of Lablab purpureus seeds
Author(s) -
de Tarso G Leopoldo Paulo,
XavierFilho J,
Lima Maria S
Publication year - 1994
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740650209
Subject(s) - lablab purpureus , lectin , glucosamine , chitin , biochemistry , sugar , galactose , chemistry , carbohydrate , biology , botany , legume , chitosan
A column of chitin was utilized to separate four lectins (hemagglutinating activity) from the seeds of ( Lablab purpureus L) Sweet. Two of these activities, which were bound by chitin and were also strongly inhibited by N ‐aeetyl‐ D ‐glucosamine, were differentiated according to their ionic properties: one is a basic (C2‐S1) and the other is an acidic (C2‐S2) lectin. A third N ‐acetyl‐ D ‐glucosamine binding lectin (Cl‐2) is also acidic in nature but showed a lower binding capacity towards this sugar than C2‐S2. A fourth lectin (C1‐1S2) was strongly inhibited by galactose and has an acidic character. The lectin is formed by subunits of 39000 and 44000 kDa.