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A comparison of the insoluble residues produced by the Klason lignin and acid detergent lignin procedures
Author(s) -
Hatfield Ronald D,
Jung HansJoachim G,
Ralph John,
Buxton Dwayne R,
Weimer Paul J
Publication year - 1994
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740650109
Subject(s) - lignin , chemistry , panicum virgatum , cellulose , forage , neutral detergent fiber , food science , botany , biology , biochemistry , bioenergy , biofuel , organic chemistry , microbiology and biotechnology
Two methods—Klason lignin (KL) and acid detergent lignin (ADL)— for determining lignin concentration in plants were compared using stem material from lucerne ( Medicago sativa L), cocksfoot ( Dactylis glomerata L) and switchgrass ( Panicum virgatum L), at three stages of maturity, and leaf samples from lucerne and cocksfoot. For all forages, KL values were higher than ADL values. Lucerne samples, which had crude protein levels twice that of the grass species, had KL values that were only 30–40% higher than ADL values; in grasses, KL values were 200–300% greater than ADL values. The addition of nitrogenous materials (bovine serum albumin, lysine, and ammonium sulfate) to commercial xylan and cellulose did not result in additional KL residue. Pyrolysis‐GC‐MS revealed that both residues appeared to be similar to the orginal plant lignin and did not appear to be contaminated with carbohydrate or protein. The higher values for grass KL residues were not due to protein con‐ tamination or incomplete hydrolysis of carbohydrates, but were more likely due to the solubilization of lignin components by the ADL treatment. KL values may give a more accurate quantification of the total lignin within forage plants.