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Relationships between processing conditions and starch and protein modifications during extrusion‐cooking of pea flour
Author(s) -
Valle Guy Della,
Quillien L,
Gueguen J
Publication year - 1994
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740640418
Subject(s) - extrusion , solubility , starch , covalent bond , pea protein , amylopectin , chemistry , legumin , food science , antigenicity , chromatography , chemical engineering , amylose , materials science , organic chemistry , biochemistry , antibody , storage protein , biology , immunology , gene , engineering , metallurgy
Pea flour was extruded under various conditions; extrusion treatment was characterised by specific mechanical energy (SME), moisture content, shear rate at the die and product temperature. Product transformation was measured by starch solubility, protein solubility in specific buffers and by immunoassay with anti‐legumin antibodies. Response surfaces showed that SME influenced starch and protein solubility more than did temperature. Decrease of protein solubility was attributed to formation of non‐covalent bonds and disulphide bonds which could partly take place at the die, according to flow conditions. The most severe extrusion conditions (SME>250 kWh t −1 ) led to the creation of other covalent bonds. Immunoassay proved an efficient method for assessing the severity of treatment by following the loss of legumin antigenicity.