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Detection of roquefortines in penicillium roqueforti isolated from moulded maize silage
Author(s) -
Ohmomo Sadahiro,
Kitamoto Hiroko Kuze,
Nakajima Tomoyoshi
Publication year - 1994
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740640210
Subject(s) - penicillium roqueforti , silage , alkaloid , thin layer chromatography , strain (injury) , biology , penicillium , food science , chemistry , botany , chromatography , anatomy
A strain (number 19) having the ability to produce some indole alkaloids was isolated from moulded maize silage, and was identical with Penicillium roqueforti. Roquefortine C was isolated from the culture broth in crystalline form as a main alkaloid and was identified as a minor alkaloid by thin‐layer chromatography (TLC) in a maize silage inoculated with the strain. Further, roquefortine A, B and D together with festuclavine were identified by TLC. The alkaloid contents in silage were analysed to be 2.40 mg kg ‐1 (roquefortine A) and 1.66 mg kg ‐1 (roquefortine C).