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β‐hydroxyacyl‐coa‐dehydrogenase (HADH) activity of unfrozen and frozen—thawed frog ( Rana esculenta ) legs
Author(s) -
Pavlov Alexander,
De Fernando Gonzalo D Garcia,
Ordoñez Juan A,
Hoz Lorenzo
Publication year - 1994
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740640202
Subject(s) - enzyme , biology , enzyme assay , incubation , chemistry , biochemistry
The β‐hydroxyacyl‐CoA‐dehydrogenase (HADH) activity of unfrozen and thawed frog legs was investigated. The enzyme was extracted by either immersing frog legs in phosphate buffer 0.1 M, pH 6.0 at 25°C for 15 min or pressing them between trichinoscopy glasses. The enzyme activity was assayed using acetoacetyl‐CoA as substrate and measured spectrophotometrically at 340 nm. It was possible by both extraction methods to distinguish between thawed and unfrozen samples although when the juice was obtained by pressing the HADH activity of the dilution was ∼ 1.5 times higher than that obtained by immersion. The HADH activity was significantly higher ( P ≤0·001) in frozen‐thawed frogs than in unfrozen legs because during freezing there is a release of HADH. No significative differences were found in the HADH activity in samples frozen in the temperature range ‐10 to ‐196°C. HADH activity was not affected by the storage time in crushed ice up to 6 days.