Endogenous amino acid flow at the terminal ileum of adult humans determined following the ingestion of a single protein‐free meal

The study sought to determine endogenous total nitrogen and amino acid flows at the terminal ileum of adult humans. Six adults (65 ± 10 kg bodyweight) with well‐established ileostomics fasted for 14 h before consuming a single protein‐free meal (240 g) containing Celite (acid‐insoluble ash) as an indigestible marker. Ileal digesta were collected for 8 h after the meal and endogenous flows were determined by reference to the marker. The mean (±SE) total 8 h output of digesta dry matter was 9.1 (± 3.51) g. The mean ileal flow of total nitrogen was 4.38 (± 0.770). with the mean amino acid flows ranging from 0.17 (± 0.009) for methionine to 2.26 (± 0.258) for glutamic acid (mg g −1 food dry matter intake).