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A comparison of silver ion HPLC plus GC with Fourier‐transform IR spectroscopy for the determination of trans double bonds in unsaturated fatty acids
Author(s) -
Toschi Tullia Gallina,
Capella P,
Holt C,
Christie W W
Publication year - 1993
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740610220
Subject(s) - double bond , fourier transform infrared spectroscopy , chemistry , fourier transform , ion , chromatography , high performance liquid chromatography , spectroscopy , organic chemistry , mathematics , chemical engineering , physics , quantum mechanics , mathematical analysis , engineering
A rapid procedure has been developed for the isolation of methyl esters of trans ‐monoenoic fatty acids by silver ion high‐performance liquid chromatography. The combined saturated plus trans ‐monoene fraction was collected for analysis by gas chromatography and comparison with the composition of the unfractionated sample. The trans ‐monoene contents of partially hydrogenated vegetable oils, determined in this way, are compared with data obtained by Fourier‐transform infrared spectroscopy. Alternative methods of base‐line correction are discussed. The infrared technique gave values that were a little higher than the chromatographic ones, presumably because the former also detected trans unsaturation in di‐ and polyunsaturated fatty acids.