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Screening for cysteine proteinase inhibitor activity in legume seeds
Author(s) -
Hines Mark E,
Osuala Chima I,
Nielsen S Suzanne
Publication year - 1992
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740590420
Subject(s) - cotyledon , phaseolus , papain , vigna , legume , lupinus , hypocotyl , biology , lima beans , botany , food science , biochemistry , enzyme
Extracts of 44 legume seeds were assayed for cysteine proteinase inhibitory (CPI) activity, using a papain/azocasein assay. Extracts with high papain inhibitory activity were further assayed with a crude gut extract (CGE) of larval Acanthoscelides obtectus (Say) (common bean weevil) against 3 H‐methemoglobin. Soybeans ( Glycine max L Merrill), lima beans ( Phaseolus lunatus L), tepary beans ( Phaseolus acutifolius A Gray), and lupine seeds ( Lupinus albus L) were found to be highest in CPI activity. Soybean hypocotyl extracts were found to have much greater CPI activity, relative to protein concentration, than cotyledon extracts. Papain was much more inhibited by soybean hypocotyl and cotyledon extracts than was A obtectus CGE. Water extracts of soybean hypocotyl flour were slightly higher in CPI activity than extracts with 0.2 M sodium phosphate buffer, pH 6.2.