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Quantitative analysis of stevioside in the leaves of Stevia rebaudiana by near infrared reflectance spectroscopy
Author(s) -
Nishiyama Paula,
Alvarez Mauro,
Vieira Luiz G E
Publication year - 1992
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740590302
Subject(s) - stevioside , stevia rebaudiana , stevia , steviol , near infrared reflectance spectroscopy , rebaudioside a , chemistry , sucrose , near infrared spectroscopy , glycoside , reflectivity , chromatography , food science , biology , organic chemistry , medicine , physics , alternative medicine , pathology , neuroscience , optics
Stevioside, a diterpenic glycoside extracted from the leaves of Stevia rebaudiana (Bert) Bertoni, is a natural sweetener 300 times sweeter than sucrose. HPLC is routinely used to measure stevioside content in Stevia leaves. Our objectives were to investigate the use of near infrared reflectance spectroscopy (NIRS) to analyse stevioside concentration in Stevia leaves. The NIRS calibrations were developed from 64 samples covering the range normally found in Stevia leaves (4‐13%). Another 30 samples were used for validation. Standard error of calibration and coefficient of multiple determination ( R 2 ) were 1.47 and 0.90 respectively, using the first derivative mathematical treatment. The validation results, although less accurate, suggest that NIRS is a precise and simple method for routine stevioside determination in Stevia leaves.