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Correction for protein content in the determination of the glucosinolate content of rapeseed by the X‐RF method
Author(s) -
Zhao Fangjie,
Evans Eric J,
Bilsborrow Paul E,
Schnug Ewald,
Syers J Keith
Publication year - 1992
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740580319
Subject(s) - glucosinolate , rapeseed , kjeldahl method , content (measure theory) , chemistry , food science , chromatography , nitrogen , analytical chemistry (journal) , brassica , mathematics , agronomy , biology , organic chemistry , mathematical analysis
Thirty six rapeseed samples from a factorial designed nitrogen and sulphur trial were analysed for total glucosinolate content by the X‐ray fluorescence (X‐RF) and HPLC methods and for protein content by the Kjeldahl method. Protein content varied from 16.0 to 22.6%. Correction for seed protein content in the calculation of glucosinolate content by X‐RF significantly improved the agreement between the two methods of glucosinolate analysis. The following equation was used in the correction: Y = 14.99 [ S (mg g −1 )‐protein %/7.5].