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Parameters affecting allicin formation as a secondary metabolite by static cultures of Allium sativum
Author(s) -
Agrawal Renu,
Mohanty Bibhudatta,
Singh Narendra S,
V Patwardhan Madhav
Publication year - 1991
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740570202
Subject(s) - allicin , callus , allium sativum , kinetin , bulb , explant culture , chemistry , sucrose , acetic acid , murashige and skoog medium , metabolite , botany , food science , biochemistry , horticulture , biology , in vitro
Garlic (Allium sativum L) bulb explants produced undifferentiated white globular callus when grown on MS medium having 2,4‐dichlorophenoxy‐acetic acid (2,4‐D) and Kinetin (Kn). Such callus when grown on medium having indole‐3‐yl‐acetic acid (IAA) and/or Kn developed into the differentiating callus which had comparatively more allicin than that of undifferentiated callus. The effect of sucrose, NH 4 + /NO 3 −ratios and hormone concentrations on growth and allicin development in the callus was investigated. There was a significant increase in allicin development in callus when grown for up to 4 weeks on medium (a) having sucrose at 3% and 5% levels; (b) having NH 4 +and NO 3 −ratios at 2:1 and 1;1; and (c) with Kn alone at a concentration of 0.5 mg litre −1 , which produced a tenfold increase in allicin content.