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An HPLc technique for flavonoid analysis in honey
Author(s) -
Ferreres Federico,
TomáasBarberáan Francisco A,
Gil MariáA I,
TomáasLorente Francisco
Publication year - 1991
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740560106
Subject(s) - galangin , kaempferol , quercetin , isorhamnetin , flavonoid , luteolin , pinocembrin , chemistry , apigenin , chromatography , acacetin , chrysin , high performance liquid chromatography , flavones , ether , organic chemistry , antioxidant
A new technique for the analysis of flavonoids in honey has been developed. This uses filtration of honey through Amberlite‐XAD‐2 and purification of the flavonoid fraction by Sephadex LH‐20. The flavonoid fraction is then analysed by HPLC. This technique allowed the identification of 16 flavonoids in honey, namely quercetin, kaempferol, 8‐methoxykaempferol, quercetin 3‐methyl ether, isorhamnetin, kaempferol 3‐methyl ether, quercetin 3,3‐dimethyl ether, quercetin 3,7‐dimethyl ether, galangin, luteolin, apigenin, genkwanin, chrysin, luteolin 7‐methyl ether, pinocembrin and pinobanksin. The flavonoids present in ten samples of honey from La Alcarria have been HPLC analysed by this technique. The fact that the flavonoid patterns are very similar, suggests that samples from other areas should be examined in order to assess if this procedure could be useful as an adjunct in studies of the geographical origin of honey.